Objective To compare the rates of entry and intake of commensal rodents for poison bait boxes made of concrete and ceramic in Shenzhen, China, and the influence of different poison baits on the rates of entry and intake of commensal rodents, and to provide a scientific basis for the purchase of poison bait boxes and poison baits for deratization in Shenzhen. Methods In July 2018, the villages of Shuiku Xincun in Luohu district and Shawei in Futian district were selected as experimental points. Forty-nine groups of poison bait boxes were placed at each experimental point. Each group consisted of one concrete box and one ceramic box. The two boxes in each group were laid 0.5 m apart according to the specifications, while the space between each two groups was 5 m. For each box, poison bait was added and replaced every day, maintaining 30 g of bromadiolone poison grains or 2 pieces of bromadiolone wax bait (15 g each). We observed and recorded regularly every day, for 9 days in Shuiku Xincun and 10 days in Shawei. Statistical analysis of the recorded data was performed using chi-square test. Results The mean rates of entry for concrete and ceramic poison bait boxes were 35.88% and 24.70%, respectively, showing a significant difference ( χ ²=27.510, P<0.001), while the mean rates of intake for concrete and ceramic poison bait boxes were 17.08% and 6.87%, respectively, also showing a significant difference ( χ ²=45.977, P<0.001). The mean rates of entry for boxes containing bromadiolone poison grains and bromadiolone wax bait were 7.55% and 11.84%, respectively, giving a significant difference ( χ ²=5.140, P=0.023), while the mean rates of intake for boxes containing the above two baits were 5.51% and 9.59%, respectively, also giving significant difference ( χ ²=5.847, P=0.016). The mean rates of entry and intake of commensal rodents for poison bait boxes in different placement positions were 53.33% vs 35.14% ( χ ²=12.547, P<0.001), and 26.67% vs 13.78% ( χ ²=10.689, P=0.001), respectively. In this experiment, the rates of entry and intake for the concrete poison bait box were better than those for the ceramic poison bait box, while the rates of entry and intake for boxes containing bromadiolone wax bait were better than those for boxes containing bromadiolone poison grains. In addition, the poison bait boxes near the rodent hole had higher rates of entry and intake than those not close to the hole. Conclusion In this experiment, when the outdoor poison bait was used in the residential area of the experimental area, the acceptability of the concrete poison bait box to commensal rodents and the rate of intake of the poison bait were significantly better than those for the ceramic poison bait box. The commensal rodents preferred the poison bait boxes containing bromadiolone wax bait during feeding process. Concrete poison bait boxes and bromadiolone wax bait should be selected as a priority in the rodent control activities in Shenzhen.

Objective To explore the correlation of monitoring methods of Bretu index, Mosq-ovitrapand “Double-mosquito net”, and evaluate the practical value of them. In order to provide the reference for choosing the proper monitoring methods in epidemic area of dengue fever. Methods Onegarden of hospital in Shenzhen was selected as the experiment site, andthe mosquito density was monitored inside by three different monitoring methodsduring October to November 2014. Results More operations are planned in epidemic area of dengue fever. There is no statistically significant correlation amongBreteau Index, Mosquito oviposition Index and Bites Index (P>0.05). Conclusion The result of Breteau Index may not be able to comprehensively evaluate the mosquito density in epidemic area of dengue fever. “Double-mosquito net” method, a kind of monitoring method for adult mosquito can be complementary.

Objective To observe the impact of water quality on the capacity of mosq-ovitrap for monitoring the density of Aedes albopictus and to evaluate the practical value of this monitoring method. Methods During July to August 2012, the peak season of Ae. albopictus, mosq-ovitraps were set in the garden of a hospital in Shenzhen, China to capture Ae. albopictus adults and eggs, and their capacities for capturing Ae. albopictus adults and eggs were compared. Results A total of 240 mosq-ovitraps were set, and 230 were recovered. The oviposition rate was 37.39%; the mosquito-trap rate was 18.26%; 51.16% of adult mosquitoes escaped. The oviposition rate was significantly higher in water from natural environment than in bottled mineral water (χ²=13.79, P<0.01). Using larva hatching as an indicator of positive oviposition response, there was a high similarity between the oviposition rate of Ae. albopictus determined using mosq-ovitraps and the actual oviposition rate (Kappa=0.828, P<0.01). The sensitivity and specificity of mosq-ovitraps for monitoring oviposition of Ae. albopictus were 82.56% and 97.92%, respectively, with a mean area under the ROC of 0.902 (range, 0.853-0.952). Conclusion Mosq-ovitrap has a good performance in monitoring the oviposition of Ae. albopictus, but its capacity for capturing adult Ae. albopictus is not satisfactory. Water quality has an impact on the performance of mosq-ovitrap in monitoring Ae. albopictus. Mosq-ovitrap has a good practical value in monitoring the oviposition of Ae. albopictus.

【Abstract】 Objective To understand the population composition of rat?shape rodents in Guangdong province and its breeding in different seasons, and to provide the theoretical basis for the control of rodents. Methods Rodents were caught daily by snap trap method in township sampled randomly monthly. The rodents captured were identified and dissected, recording the pregnancy situation. Results The trapping rate of rat?shape rodents was 9.98% in the residential areas, including 4.98% of rats. It included 48.73% Rattus norvegicus, 15.50% R.tanezumi mice, 35.17%Suncus murinus and 0.59% Mus musculus. The average pregnancy rate of R.norvegicus was 4.06% and the average litter size was 5.35. The average pregnancy rate of R.tanezumi was 5.63%  and  the  average  litter  size  was  2.76.  That  of  Suncus  murinus  was  15.00%  and  its  litter size was 3.32. Conclusion R.norvegicus and Suncus murinus are the dominant species in the residential areas in township. M.musculus is the rare specie. The breeding seasons of R.norvegicus and R.tanezumi are in spring and autumn, and the breeding peak in spring is higher than that in the autumn. It has also a small reproduction peak in August. However, Suncus murinus can breed all year round, and there are also two peaks. One peak is in March?June, the other is in October?November. It should strengthen the killing of rodents in spring and autumn.

Objective To study the dynamic changes of murine-like-animal community in Zhanjiang,Guangdong from 1950 to 2000. Methods Data of plague surveillance in Zhanjiang during the past 50 years were collected and sorted out to describe and analyze the changes of murine-like-animal community by year. Results A total of 237 831 murine-like-animals were captured and identified,including 11 species of three genera in two orders. Totally,157 412 murine-like-animals in four species of three genera were captured domestically,with Rattus flavipectus as dominant species,accounting for 68% of the total,and Suncus murinus and Rattus norvegicus as common species,accounting for 25% and 6%,respectively. A total of 80 419 wild murine-like-animals in eight species of two genera in two orders were captured,with R.losea and Bandicoot indicia as dominant species,accounting for 80% and 18% of the total,respectively,and R.rattus hainanicus and Suncus murinus as common species. Conclusion In domestic rats of urban areas, R.flavipectus had been replaced by R.norvegicus which had become a dominant species there,but in rural areas, R.flavipectus was still the dominant species. Among wild rodents,population of R.losea reduced yearly,but that of Bandicota indica increased yearly.

Objective:To observe the current situation of the HFRS Epidemic Foci in Shenzhen.Methods:11 places were selected to lay cages for capturing mice;the population structure of rat was investigated through cluster sampling,the anti-Hantavirus antibody was detected by Indirect Immunofluorescent Assay and Hantavirus antigen was detected by Direct Immunofluorescent Assay.Results:625 mouse-like animals were captured and the capture rate was 11.07%,in which there were 368 Rattus norvegicus(58.88%),and the serum positive rate of anti-Hantavirus antibody was 7.17%(36/502),GMT was 54.45,the antigen positive rate of mouse lung was 0.81%(5/619),and all the positive serum and lung were from Rattus norvegicus.Conclusions:There are rattus-related HFRS Epidemic Foci in Shenzhen distributed in all the 6 districts of the city and 11 investigated places,and the Rattus norvegicus are the major host animals.

Objective:To investigate the population,constitution and distribution of rat and tick,flea,gamasid and jigger on its body surface in Shenzhen.Methods:11 places were selected to capture mice at the populated areas,farming areas and mountain areas through chuster sampling;the captured mice was identified and then,the insects on their body surfaces were collected to calculate the insect(tick,flea,gamasid and jigger) carrying rate and index.Results:625 mouse-like animals were captured and the capture rate was 11.07%,in which 560 were Order Rodentia animals and they belong to 1 family,3 genera and 10 species,and 65.71% were Rattus norvegicus;65 were Order Insectivorora animals and they belong to 1 family,1 genus and 1 species.Ticks belong to 2 genera and 2 species and 87.29% were Ixodes granulatus;Fleas belong to 3 genera and 3 species,98.56% of them were Xenopsylla cheopis;Gamasids belong to 2 genera and 3 species and 91.94% were Laelaps echidninus;Jiggers belong to 4 genera and 6 species and 27.12% were Walchia xishaensis;The insect carrying rates of the mice were 7.09%(tick),9.29%(flea),25.37%(gamasid) 7.40%(jigger) and the index were 0.17,0.30,0.80,1.28 respectively.Conclusions:The dominant rat at populated areas is Rattus norvegicus;The dominant rat at farming areas is Rattus norvegicus,Rattus losea and Rattus flavipectus;The dominant rat at mountain areas is Rattus norvegicus and Rattus sladeni;Ixodes granulatus,Xenopsylla cheopis,Laelaps echidninus and Walchia xishaensis are the dominant strains of tick,flea,gamasid and jigger respectively;their major host are Rattus sladeni,Rattus flavipectus,Rattus losea and Rattus sladeni respectively.

A survey of animal reservoir of Lyme disease was conducted in June,1996.49 mice and 250 ticks were tested by isolation using BSK medium.A strain of spirochete (LPR ₃₀) was isolated from the embryo of rat ( Rattus edwerdsi).Characterization of the spirochete by microscopy and serology (IFA) using genusand speciesspecific monoclonal antibodies (H ₅₃₃₂、H _{9724、H6831),confirmed that it is a strain of Borrelia burgdorferi. }